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 SCI 291 - Life Science Laboratory

Content

 

This course is highly recommended for all life science majors and medical track students, but designed and also recommended for a UCR science student. It is a great introduction to the biochemical and molecular laboratory.

There are five basic themes or units, each characteristic or representative of classical clinical and biomedical research. Additional clinical diagnostic themes are added whenever there is an opportunity.

•             [1] Blood 

•             [2] Proteins

•             [3] Bacteria, hygiene and Genetically Modified Organisms (GMOs)

•             [4] DNA

•             [5] Bioinformatics

The concepts, principles and techniques used in this course regarding blood and blood proteins include a basic blood cell analysis (hemogram), protein electrophoresis, enzyme-linked immunosorbent assay (ELISA) and a patient case. 

With respect to bacteria, a series of tests will be carried out to check hygiene and sterilization procedures, bacterial species determination and the effect of antibiotics on bacterial growth. It also includes engineering bacteria to express proteins of interest using safe microbiological techniques. Students then extract and isolate the protein of interest, Green Fluorescent Protein (GFP). The purity of the isolated protein is further analyzed using SDS-PAGE.

Regarding DNA, students will isolate their own DNA and use polymerase chain reaction (PCR) and gel electrophoresis to analyze their inheritance of a particular sequence of DNA. Additionally, students will practice performing other common techniques used in most life science laboratories to assess DNA.

In addition to the experimental labs, one computer lab is dedicated to becoming acquainted with some simulated (in silico) procedures, including data mining (DNA and protein data base searches). 

Instructor

Dr. Theresa Dinse and Dr. Frans van Overveld

Track

Laboratory

Prerequisites

The following courses are required in order to take this course:

  • SCI 131 Introduction to Life Science
  • SCI 191 Introductory Science Laboratory

Required for

This course is required in order to take the following course

  • ​SCI 391 Advanced Science Laboratory​